On a different note, I finally started learning my way around the lab. The names of the equipment are apt; the vortexer mixes solutions in vials by creating a mini vortex, and the sonicator mixes solutions in submerged vials by sending sound waves through water (and, as the person training me said, it literally sounds like a dying bat). But don't let this simplicity fool you. Even though the name may be easy, using the equipment is not. Especially when the equipment you're using has, for all intents and purposes, a pressurized cannon connected to it. For those skeptics out there, I give you this proof:
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No, it's not a death ray, although it looks like one. It's called a rotavapor. |
That tank is filled with compressed nitrogen. It's chained to the wall because, if it fell over, it'd literally blow through a wall or two. Or maybe three. I'm actually going to be using this piece of equipment (yay, lucky me). The rotavapor rotates the vial while pumping in nitrogen gas, allowing the solvent to evaporate off and leaving the lipid evenly distributed. I think I'll post about my experiment process later, since it's rather lengthy. However, I will say that working in a lab requires patience. Preparing the liposomes took hours, and the actual test at the end took minutes. Most of those minutes, furthermore, were all controls, so those results didn't mean much, since they were expected.
I've enjoyed working in the lab so far. I'm a bit nervous for next week, however. My uncle says I'm going to replicate this mostly by myself, although I will receive help if I ask for it. My uncle is also the one that pushed me to take the bus. I think his plan is to make me more independent (and it might actually be working). Maybe I'll use this independence to walk over to the UVU library. Unfortunately, I don't think the library will allow me to check out any books unless I have a card - which I don't have. But my cousin has one....
Until next time!
- Lauren
Wow your research and life experiences are getting real! Are there any machines you can't use? Which ones are the hardest to use?
ReplyDeleteAnd of course, the most pressing question, will you be getting a library card?
There's a lot of machines I can't use, mostly because I've never been introduced to them. I could probably walk past them and not know I was missing a machine. I'm mostly not allowed to work with animals or extremely dangerous chemical, due to liability reasons. I think the hardest machines to use are actually the pipettes/graduated cylinders, since it's frustrating getting everything exact.
DeleteInstead of getting my own library card, I might just "borrow" my cousin's for a month.
Hi Laur: This is looking like it is going to be such a great experience for you both in the lab and personally. You are working with equipment I have never heard of so, I will be following your lab experience with great interest to learn myself.
ReplyDeleteSounds like tons of fun! Hows the weather by the way, is that jacket coming in handy? And what exactly are you going to be using the death ray, I mean rotavapor for?
ReplyDeleteI haven't worn the jacket yet, since the weather has been "nice" (I still find it cold, of course), but that might change later this week. My phone says it'll snow more.
DeleteWe use the rotavapor to dry out the lipids evenly in a vial, since they're in a chloroform solution. After we dry them out, then we can ad internal buffer and mix them up again so they form liposomes.
Hey Lauren, how exactly do you make the liposomes? Do you just use the rotovapor on some source of phospholipids, or is there some other way you're creating the liposomes?
ReplyDeleteMy whole project is centered on making these liposomes. I was going to post my methods in a later week (it's rather complicated and I don't think I understand everything yet), but if people are wondering what, exactly, it is that I'm doing, maybe I should make that my next post....
DeleteIt sounds like you are working with some really cool equipment. Is it hard to learn how to use it?
ReplyDeleteIt's not particularly difficult, I just have to get over my fear that I'll break everything.
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